My Profile

EDUCATION

Ph.D. in Marine Life Science (Reading)

CRISPR / Cas9 gene editing in fish for development of Aquaculture

March 03, 2021 – Present

 College of Marine Life Science, Jeju National University, Republic of Korea.


M.sc in Preventive Veterinary Medical science  (Research based/Two years)

February 28, 2017 – February 25, 2019

College of Veterinary Medicine, Chungnam National University, Republic of Korea.

 

GPA

: 4.33 / 4.5

Dissertation

: RING finger protein 178 negatively regulates type I  

  interferon signaling pathway

 

B.sc. (Special) in Fisheries and Marine sciences (English medium/ Four-year degree)

November 03, 2009 – December 30, 2014

 

Faculty of Fisheries and Marine sciences & Technology, University of Ruhuna, Matara

 

Field of Specialization

: Aquaculture

Class

GPA

: Second class (upper division)

: 3.38/4

Dissertation

: Production level, consumer preference of bivalves and    

  Potential for production of sauce as a value added product

 

LABORATORY SKILLS



 

Field of Microbiology, Molecular biology, Genetics,  immunology and CRISPR/Cas9 gene editing

 

Microbiology,

·         Gram staining,

·         Selective and deferential media test,

·         Antibiotics test,

 

 

 

 

 

 

 

·         ELISA,

·         Native PAGE,

·         SDS/PAGE,

·         Western blotting,

·         Immunoprecipitation,

·         Phosphorylation/ Ubiquitination assay,

·         Dimerization / Oligomerization assay

·         Confocal microscopy assay,

·         Luciferase assay,

·         RNA extraction,

·         cDNA synthesizing,

 

 

 

 

 

 

 

·         Protein induction

·         Virus infection to cells,

·         Cell viability assay,

·         Virus amplification,

·         Plaque assay,

·         TCID50,

·         MTT assay,

·         Hemagglutination assay,

 

Molecular biology and genetics

·         PCR

·         qRT-PCR,

·         Expression vector Cloning,

·         DNA extraction (Mini prep, Midi prep, Maxi prep and CsCl2 prep),

·         Agarose gel electrophoresis

·         Cell culture,

·         Gene transfection to cells,

 

 


CRISPR/Cas9 gene editing (Eukaryotes and Prokaryote )

·         gRNA and Cri- primer designing for the target gene

·         gRNA synthesis

·         Micro injection

·         T7E1 assay for the genotyping

·         Chromatograph analysis for the genotyping


Field of Fisheries and Aquaculture

 

 

·         Fish handling

·         Fish feed preparation,

·         Water quality analysis,

·         Fresh water fish Breeding,

·         Analysis of Proximate compositions,

·         Identification of fish diseases and treatments

·         Fish dissection

·         Histological slide preparation,

·         Postmortem analysis for dynamite fishing

·         Identification of fin fish and shellfish up to species level using identification keys

 


PROFESSIONAL EXPERIENCE

Research Scientist

March 03, 2019 – Present

Laboratory of Marine Molecular Genetics, Marine Science institute, Jeju National University, Republic of Korea.

·         Laboratory analysis and investigations

·         Maintaining Zebrafish rearing system


Lecturer (Probationary)  (One year and nine months)

April 24, 2019 – February 14, 2021

Department of Aquaculture and Aquatic Resources Management, University College of Anuradhapura, Sri Lanka.

·         Lectured, directed, and taught advanced lessons, provided presentation support, trained apprentices, and developed courses’ material and tools

·         Advised and supervised students in their research work and complex assignments

·         Invigilating examinations

·         Exam paper moderation

·         Preparation of practical classes and handouts

·         Demonstrate for practical classes

·         Arrangement of field visits

 

Research assistant (Two years)

February 28, 2017 – February 25, 2019

Laboratory of Microbiology, College of veterinary medicine, Chungnam National University, Republic of Korea.

 

Temporary Lecturer (One year)

February 18, 2016 – February 18, 2017

Department of Fisheries & Aquaculture, Faculty of Fisheries and Marine sciences and Technology, University of Ruhuna, Sri Lanka

·         Lecture notes preparation

·         Lecturing

·         Invigilating examinations

·         Checking and assessing students' works

·         Preparation of practical classes

·         Demonstrate for practical classes

·         Arrangement of field visits

 

Temporary Demonstrator (Nine months)

May 15, 2015 – February 18, 2016

Department of Fisheries & Aquaculture, Faculty of Fisheries and Marine sciences and Technology, University of Ruhuna, Sri Lanka

·         Demonstrate for practical classes

·         Checking and assessing students' works

·         Invigilating examinations

·         Guide students in field visits

·         Participate in laboratory analysis

 

Trainee Quality control supervisor /Internship (One month)

January 01, 2015 - February 28, 2015

Taprobane Seafood (PVT) LTD, Bubulla watte, Haldaduwana, Dankotuwa, Sri Lanka

·         Quality controlling of raw and processed fish, shrimps and crab meat

·         Production analysis of processed fish, shrimps and crab meat

·         Documentation


Assistant Aquaculturist (One year)

October 20, 2012 - December 13, 2013

Water World Aquarist (PVT) LTD, Silverdale estate, Kakanadura, Matara, Sri Lanka

·         Ornamental fish breeding and rearing

·         Pond management

·         Packing of live fish

·         Documentation and accounting

 

OTHER POSITIONS HELD

2020-Coordinator of Industrial Advisory board - University College of Anuradhapura, Sri Lanka

2019-Safeguard officer - University College of Anuradhapura, Sri Lanka.

2019-Coordinator of Annual symposium - University College of Anuradhapura, Sri Lanka.


FOREIGN TRAINING AND WORKSHOPS ATTENDED

International Training Course on “ Detection of Environmental Pollutants and Monitoring of Health Effects”

February 24, 2020 – March 06, 2020 (02 weeks)

Organized by Chulabhorn Research Institute, Bangkok, THAILAND

 

LOCAL TRAINING AND WORKSHOPS ATTENDED

Staff development programme for probationary lecturers (Cycle 22)

February 28, 2020 – January 29, 2021 (02 days)

Organized by Staff Development Centre, University of Kelaniya.

Training program on Ornamental Fish breeding

February 01, 2016 – February 02, 2016 (02 days)

Organized by National Aquaculture Development Authority (NAQDA), Ginigathhena

Marine chart reading communication and operation of satellite navigation

August 04, 2012 – January 03, 2013 (15 days)

National Institute of Fisheries & Nautical Engineering, Galle.

Successfully completed

Workshop on “Marine Science and Disaster Management”

September 10, 2012 – September 11, 2012 (02 days)

Organized by National Institute of Fisheries & Nautical Engineering, Mattakkuliya.

Successfully completed


AWARDS

Best poster presenter

KOFFST International Conference 2021. Busan: Korean Federation of Fisheries Science and Technology Studies.

Characterization and expression analysis of Suppressor of Cytokine sign aling 5b (SOCS5b) from Liza haematochelila (Mullet).


ADDITIONAL COMPETENCIES

Proficiency in English Final Certificate

2010 to 2013

University of Ruhuna, Sri Lanka

Successfully completed

Computer literacy course

2009 – 2010                                                    

Department of Computer Science, Faculty of Science, University of Ruhuna, Sri Lanka

Successfully completed

Diploma in Information Technology with E-Commerce*

January 25, 2009 – May 20, 2009

ESOFT Computer Studies PVT LTD, No 158F 1/1 Galle road, Colombo 4, Sri Lanka

Successfully completed.


SKILLS PROFILE

·         Computer skill

·         Language ability - Fluent in Sinhala (native tongue), Proficient in English

·         Sport - Chess

·         Driving skill

Date of issued April 04, 2007 (Department of Motor Traffic Sri Lanka)

All cars, Dual purpose Vehicles and Motorcycles

·         Music - Singing, Bass/ Rhythm/ Lead/ Box guitar playing/Keyboard

·         Painting






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